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biotin coupled antibodies against cd33  (Thermo Fisher)


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    Thermo Fisher biotin coupled antibodies against cd33
    Hotairm1 binding to S100A9 protein in MDSCs from septic patients. PBMCs were isolated from early and late septic patients and depleted of the HLA-DR + cells using biotin-coupled anti-HLA-DR antibody and anti-biotin microbeads, followed by the positive selection of <t>CD33</t> + LOX1 + cells with biotin-coupled <t>anti-CD33,</t> anti-CD11b, and anti-LOX1 antibodies. (A) The CD33 + CD11b + LOX1 + HLA-DR- cells were fixed in 0.2% formaldehyde to preserve RNA-protein complexes. The whole cell lysate was incubated with 5 μg of anti-S100A9 or anti-IgG isotype control antibody and then cross-linked to protein G magnetic beads overnight at 4°C. RNA was extracted from the immunoprecipitated protein-RNA complexes by Trizol reagent and used for detecting Hotairm1 by RT-qPCR, using human Hotairm1 primer assay. (B) CD33 + CD11b + LOX1 + HLA-DR- cells from late septic patients were transfected with scramble or Hotairm1 siRNA for 36 h. The cells were treated as described in A , and RT-PCR determined levels of Hotairm1. (C) Whole cell lysate of CD33 + CD11b + LOX1 + HLA-DR-cells were cleared by centrifugation and subjected to immunoprecipitation with S100A9 or IgG isotype control antibody as described in A . The immunoprecipitated protein complexes were assessed by immunoblotting using phospho-S100A9 (Thr113) antibody. The membrane was stripped and reprobed with a phospho-p38 antibody. The results are representative of two experiments. (D) CD33 + CD11b + LOX1 + HLA-DR-cells were transfected with scramble or Hotairm1 siRNA for 36 h, then washed and incubated with 1 μg/ml of bacterial LPS for 12 h. ELISA determined the levels of IL-10 in the culture supernatants. The data are mean ± SD of 5 patients per group.*p < 0.05, vs . early septic or control siRNA; **p < 0.05, vs . Hotairm1 siRNA/early septic; #p < 0.05, vs . control siRNA.
    Biotin Coupled Antibodies Against Cd33, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2656 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin coupled antibodies against cd33/product/Thermo Fisher
    Average 99 stars, based on 2656 article reviews
    biotin coupled antibodies against cd33 - by Bioz Stars, 2026-03
    99/100 stars

    Images

    1) Product Images from "Hotairm1 Controls S100A9 Protein Phosphorylation in Myeloid-Derived Suppressor Cells during Sepsis"

    Article Title: Hotairm1 Controls S100A9 Protein Phosphorylation in Myeloid-Derived Suppressor Cells during Sepsis

    Journal: Journal of clinical & cellular immunology

    doi:

    Hotairm1 binding to S100A9 protein in MDSCs from septic patients. PBMCs were isolated from early and late septic patients and depleted of the HLA-DR + cells using biotin-coupled anti-HLA-DR antibody and anti-biotin microbeads, followed by the positive selection of CD33 + LOX1 + cells with biotin-coupled anti-CD33, anti-CD11b, and anti-LOX1 antibodies. (A) The CD33 + CD11b + LOX1 + HLA-DR- cells were fixed in 0.2% formaldehyde to preserve RNA-protein complexes. The whole cell lysate was incubated with 5 μg of anti-S100A9 or anti-IgG isotype control antibody and then cross-linked to protein G magnetic beads overnight at 4°C. RNA was extracted from the immunoprecipitated protein-RNA complexes by Trizol reagent and used for detecting Hotairm1 by RT-qPCR, using human Hotairm1 primer assay. (B) CD33 + CD11b + LOX1 + HLA-DR- cells from late septic patients were transfected with scramble or Hotairm1 siRNA for 36 h. The cells were treated as described in A , and RT-PCR determined levels of Hotairm1. (C) Whole cell lysate of CD33 + CD11b + LOX1 + HLA-DR-cells were cleared by centrifugation and subjected to immunoprecipitation with S100A9 or IgG isotype control antibody as described in A . The immunoprecipitated protein complexes were assessed by immunoblotting using phospho-S100A9 (Thr113) antibody. The membrane was stripped and reprobed with a phospho-p38 antibody. The results are representative of two experiments. (D) CD33 + CD11b + LOX1 + HLA-DR-cells were transfected with scramble or Hotairm1 siRNA for 36 h, then washed and incubated with 1 μg/ml of bacterial LPS for 12 h. ELISA determined the levels of IL-10 in the culture supernatants. The data are mean ± SD of 5 patients per group.*p < 0.05, vs . early septic or control siRNA; **p < 0.05, vs . Hotairm1 siRNA/early septic; #p < 0.05, vs . control siRNA.
    Figure Legend Snippet: Hotairm1 binding to S100A9 protein in MDSCs from septic patients. PBMCs were isolated from early and late septic patients and depleted of the HLA-DR + cells using biotin-coupled anti-HLA-DR antibody and anti-biotin microbeads, followed by the positive selection of CD33 + LOX1 + cells with biotin-coupled anti-CD33, anti-CD11b, and anti-LOX1 antibodies. (A) The CD33 + CD11b + LOX1 + HLA-DR- cells were fixed in 0.2% formaldehyde to preserve RNA-protein complexes. The whole cell lysate was incubated with 5 μg of anti-S100A9 or anti-IgG isotype control antibody and then cross-linked to protein G magnetic beads overnight at 4°C. RNA was extracted from the immunoprecipitated protein-RNA complexes by Trizol reagent and used for detecting Hotairm1 by RT-qPCR, using human Hotairm1 primer assay. (B) CD33 + CD11b + LOX1 + HLA-DR- cells from late septic patients were transfected with scramble or Hotairm1 siRNA for 36 h. The cells were treated as described in A , and RT-PCR determined levels of Hotairm1. (C) Whole cell lysate of CD33 + CD11b + LOX1 + HLA-DR-cells were cleared by centrifugation and subjected to immunoprecipitation with S100A9 or IgG isotype control antibody as described in A . The immunoprecipitated protein complexes were assessed by immunoblotting using phospho-S100A9 (Thr113) antibody. The membrane was stripped and reprobed with a phospho-p38 antibody. The results are representative of two experiments. (D) CD33 + CD11b + LOX1 + HLA-DR-cells were transfected with scramble or Hotairm1 siRNA for 36 h, then washed and incubated with 1 μg/ml of bacterial LPS for 12 h. ELISA determined the levels of IL-10 in the culture supernatants. The data are mean ± SD of 5 patients per group.*p < 0.05, vs . early septic or control siRNA; **p < 0.05, vs . Hotairm1 siRNA/early septic; #p < 0.05, vs . control siRNA.

    Techniques Used: Binding Assay, Isolation, Selection, Incubation, Control, Magnetic Beads, Immunoprecipitation, Quantitative RT-PCR, Transfection, Reverse Transcription Polymerase Chain Reaction, Centrifugation, Western Blot, Membrane, Enzyme-linked Immunosorbent Assay



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    biotin coupled antibodies against cd33  (Thermo Fisher)
    99
    Thermo Fisher biotin coupled antibodies against cd33
    Hotairm1 binding to S100A9 protein in MDSCs from septic patients. PBMCs were isolated from early and late septic patients and depleted of the HLA-DR + cells using biotin-coupled anti-HLA-DR antibody and anti-biotin microbeads, followed by the positive selection of <t>CD33</t> + LOX1 + cells with biotin-coupled <t>anti-CD33,</t> anti-CD11b, and anti-LOX1 antibodies. (A) The CD33 + CD11b + LOX1 + HLA-DR- cells were fixed in 0.2% formaldehyde to preserve RNA-protein complexes. The whole cell lysate was incubated with 5 μg of anti-S100A9 or anti-IgG isotype control antibody and then cross-linked to protein G magnetic beads overnight at 4°C. RNA was extracted from the immunoprecipitated protein-RNA complexes by Trizol reagent and used for detecting Hotairm1 by RT-qPCR, using human Hotairm1 primer assay. (B) CD33 + CD11b + LOX1 + HLA-DR- cells from late septic patients were transfected with scramble or Hotairm1 siRNA for 36 h. The cells were treated as described in A , and RT-PCR determined levels of Hotairm1. (C) Whole cell lysate of CD33 + CD11b + LOX1 + HLA-DR-cells were cleared by centrifugation and subjected to immunoprecipitation with S100A9 or IgG isotype control antibody as described in A . The immunoprecipitated protein complexes were assessed by immunoblotting using phospho-S100A9 (Thr113) antibody. The membrane was stripped and reprobed with a phospho-p38 antibody. The results are representative of two experiments. (D) CD33 + CD11b + LOX1 + HLA-DR-cells were transfected with scramble or Hotairm1 siRNA for 36 h, then washed and incubated with 1 μg/ml of bacterial LPS for 12 h. ELISA determined the levels of IL-10 in the culture supernatants. The data are mean ± SD of 5 patients per group.*p < 0.05, vs . early septic or control siRNA; **p < 0.05, vs . Hotairm1 siRNA/early septic; #p < 0.05, vs . control siRNA.
    Biotin Coupled Antibodies Against Cd33, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin coupled antibodies against cd33/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    biotin coupled antibodies against cd33 - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier

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    Hotairm1 binding to S100A9 protein in MDSCs from septic patients. PBMCs were isolated from early and late septic patients and depleted of the HLA-DR + cells using biotin-coupled anti-HLA-DR antibody and anti-biotin microbeads, followed by the positive selection of CD33 + LOX1 + cells with biotin-coupled anti-CD33, anti-CD11b, and anti-LOX1 antibodies. (A) The CD33 + CD11b + LOX1 + HLA-DR- cells were fixed in 0.2% formaldehyde to preserve RNA-protein complexes. The whole cell lysate was incubated with 5 μg of anti-S100A9 or anti-IgG isotype control antibody and then cross-linked to protein G magnetic beads overnight at 4°C. RNA was extracted from the immunoprecipitated protein-RNA complexes by Trizol reagent and used for detecting Hotairm1 by RT-qPCR, using human Hotairm1 primer assay. (B) CD33 + CD11b + LOX1 + HLA-DR- cells from late septic patients were transfected with scramble or Hotairm1 siRNA for 36 h. The cells were treated as described in A , and RT-PCR determined levels of Hotairm1. (C) Whole cell lysate of CD33 + CD11b + LOX1 + HLA-DR-cells were cleared by centrifugation and subjected to immunoprecipitation with S100A9 or IgG isotype control antibody as described in A . The immunoprecipitated protein complexes were assessed by immunoblotting using phospho-S100A9 (Thr113) antibody. The membrane was stripped and reprobed with a phospho-p38 antibody. The results are representative of two experiments. (D) CD33 + CD11b + LOX1 + HLA-DR-cells were transfected with scramble or Hotairm1 siRNA for 36 h, then washed and incubated with 1 μg/ml of bacterial LPS for 12 h. ELISA determined the levels of IL-10 in the culture supernatants. The data are mean ± SD of 5 patients per group.*p < 0.05, vs . early septic or control siRNA; **p < 0.05, vs . Hotairm1 siRNA/early septic; #p < 0.05, vs . control siRNA.

    Journal: Journal of clinical & cellular immunology

    Article Title: Hotairm1 Controls S100A9 Protein Phosphorylation in Myeloid-Derived Suppressor Cells during Sepsis

    doi:

    Figure Lengend Snippet: Hotairm1 binding to S100A9 protein in MDSCs from septic patients. PBMCs were isolated from early and late septic patients and depleted of the HLA-DR + cells using biotin-coupled anti-HLA-DR antibody and anti-biotin microbeads, followed by the positive selection of CD33 + LOX1 + cells with biotin-coupled anti-CD33, anti-CD11b, and anti-LOX1 antibodies. (A) The CD33 + CD11b + LOX1 + HLA-DR- cells were fixed in 0.2% formaldehyde to preserve RNA-protein complexes. The whole cell lysate was incubated with 5 μg of anti-S100A9 or anti-IgG isotype control antibody and then cross-linked to protein G magnetic beads overnight at 4°C. RNA was extracted from the immunoprecipitated protein-RNA complexes by Trizol reagent and used for detecting Hotairm1 by RT-qPCR, using human Hotairm1 primer assay. (B) CD33 + CD11b + LOX1 + HLA-DR- cells from late septic patients were transfected with scramble or Hotairm1 siRNA for 36 h. The cells were treated as described in A , and RT-PCR determined levels of Hotairm1. (C) Whole cell lysate of CD33 + CD11b + LOX1 + HLA-DR-cells were cleared by centrifugation and subjected to immunoprecipitation with S100A9 or IgG isotype control antibody as described in A . The immunoprecipitated protein complexes were assessed by immunoblotting using phospho-S100A9 (Thr113) antibody. The membrane was stripped and reprobed with a phospho-p38 antibody. The results are representative of two experiments. (D) CD33 + CD11b + LOX1 + HLA-DR-cells were transfected with scramble or Hotairm1 siRNA for 36 h, then washed and incubated with 1 μg/ml of bacterial LPS for 12 h. ELISA determined the levels of IL-10 in the culture supernatants. The data are mean ± SD of 5 patients per group.*p < 0.05, vs . early septic or control siRNA; **p < 0.05, vs . Hotairm1 siRNA/early septic; #p < 0.05, vs . control siRNA.

    Article Snippet: Next, the remaining cells were subjected to positive selection using biotin-coupled antibodies against CD33 (Cat #MA1-19522; Invitrogen, Waltham, MA), CD11b (Cat #130-113-795), and LOX-1 (Cat #130-122-119; both from Milteny Biotec).

    Techniques: Binding Assay, Isolation, Selection, Incubation, Control, Magnetic Beads, Immunoprecipitation, Quantitative RT-PCR, Transfection, Reverse Transcription Polymerase Chain Reaction, Centrifugation, Western Blot, Membrane, Enzyme-linked Immunosorbent Assay